Works to correct defects on the Great Ouse viaduct are due to be carried out this weekend.
Work will start from the early hours of Sunday, 14 June through to 4am on Monday, 15 June.
The Busway as well as pedestrian and cycle access will be closed between the St Ives and Swavesey section during this time so works can be completed safely and quickly.
Buses will follow a diversion route which will mean the Fen Drayton Lakes stop will not be served. However, all other stops will continue to be used.
The Busway will be closed after the last bus on Saturday night and should re-open on Monday morning from 4am.
Traffic management will be in place at St.Ives and also at the Fen Drayton stop area.